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Vitamin D fortification in milk uses a fixed amount per quantity of milk to ensure consistent intake. The established level is 400 International Units per quart of milk, which means about 100 IU per 8-ounce cup. This standard applies regardless of the milk’s fat content, so the fortification amount remains the same per quart, not per quart of butterfat. Therefore, 400 IU per quart is the correct amount.